Development and Validation of an HPLC Method for the Characterization and Assay of the Saponins from Ilex paraguariensis A. St.-Hil (Mate)Fruits
Material type:
TextSeries: ; Journal of Liquid Chromatography & Related Technologies, 30(1), p.87-95, 2007Contained works: - Pavei, C
- Guzatto, P
- Ros Petrovick, P
- Gosmann, G
- González-Ortega, G
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Two improved HPLC methods for the characterization and assay of the saponin content in green fruits of Ilex paraguariensis (erva-mate)are described. Both HPLC methods consisted of isocratic separation at room temperature, using a RP-18 column with UV-Vis detection. The first system was intended for the characterization of the main I. paraguariensis saponin fraction, while the second one was specifically developed and validated for its quantitation. Matesaponin-3 was used as external standard. From the freeze dried extract, ten peaks were characterized as saponins by TLC and HPLC analysis. The matesaponin-3 content was 5.13 g
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