CICY GOBIERNO DE MÉXICO · SECIHTI

BIBLIOTECA

CICY.mx › Biblioteca › Catálogo en línea

Expression of a Foreign Gene in Chlamydomonas reinhardtii Chloroplast

Material type: TextSeries: ; Journal of BioScience and BioEngineering, 87(3), p.307-314, 1999Contained works:
  • Ishikura, K
  • Takaoka, Y
  • Kato, K
  • Sekine, M
  • Yoshida, K
Subject(s): Online resources: Abstract: Chimeric genes for expression of a foreign gene in the Chlamydomonas reinhardtii chloroplast were constructed. These chimeric genes are composed of the promoter from chloroplast genes, rbcL, psbA, and atpA, S- and S/-untranslated regions, and the Escherichia coli ,9-glucuronidase (GUS)structural gene (uidA)as a foreign gene. Three types of chloroplast transformants (RG, PG, and AG), which contained the rbcL-uidA, psbA-uidA, and atpA-uidA chimerlc genes integrated in the chloroplast genome, were generated by particle bombardment. The AG transformant grown under photoautotrophlc conditions showed the highest GUS activity (130 nmol/min/mg protein)so far reported in C. reinhurdtii, and the accumulated GUS protein accounted for 0.08
Tags from this library: No tags from this library for this title. Log in to add tags.
Star ratings
    Average rating: 0.0 (0 votes)
Holdings
Cover image Item type Current library Home library Collection Shelving location Call number Materials specified Vol info URL Copy number Status Notes Date due Barcode Item holds Item hold queue priority Course reserves
REF1 CICY F1 B-9793 (Browse shelf(Opens below)) Available

Chimeric genes for expression of a foreign gene in the Chlamydomonas reinhardtii chloroplast were constructed. These chimeric genes are composed of the promoter from chloroplast genes, rbcL, psbA, and atpA, S- and S/-untranslated regions, and the Escherichia coli ,9-glucuronidase (GUS)structural gene (uidA)as a foreign gene. Three types of chloroplast transformants (RG, PG, and AG), which contained the rbcL-uidA, psbA-uidA, and atpA-uidA chimerlc genes integrated in the chloroplast genome, were generated by particle bombardment. The AG transformant grown under photoautotrophlc conditions showed the highest GUS activity (130 nmol/min/mg protein)so far reported in C. reinhurdtii, and the accumulated GUS protein accounted for 0.08

There are no comments on this title.

to post a comment.