Characteristics of growth and tropane alkaloid synthesis in Atropa belladonna roots transformed by Agrobacterium rhizogenes
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TextSeries: ; Journal of Biotechnology, 16(3-4), p.171-186, 1990Contained works: - Sharp, J.M
- Doran, P.M
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Growth of Atropa belladonna hairy roots in shake flasks followed first-order kinetics. The specific growth rate was 0.28 d-1, corresponding to a doubling time of 60 h. In airlift reactors, first order kinetics were not observed due to nutrient limitation. Synthesis of tropane alkaloids was growth-associated; no atropine was released into the medium. Sugar concentrations were analyzed during hairy-root culture for calculation of atropine yield. Atropine yields from substrate were 1.4 mg g-1 in shake flasks and 0.46 mg g-1 in airlift reactors. Although levels of atropine in reactor-grown roots (0.37
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