Combination of Suspension Array and Mycelial Growth Assay for Detecting Multiple-Fungicide Resistance in Botrytis cinerea in Hebei Province in China. (Record no. 27889)
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| 000 -LEADER | |
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| campo de control de longitud fija | 03398nam a2200337Ia 4500 |
| 003 - IDENTIFICADOR DE NÚMERO DE CONTROL | |
| campo de control | MX-MdCICY |
| 005 - FECHA Y HORA DE LA ÚLTIMA TRANSACCIÓN | |
| campo de control | 20260521091609.0 |
| 040 ## - FUENTE DE CATALOGACIÓN | |
| Centro/agencia transcriptor | CICY |
| 090 ## - LOCALMENTE ASIGNADO TIPO-LC NÚMERO DE CLASIFICACIÓN (OCLC); NÚMERO DE CLASIFICACIÓN LOCAL (RLIN) | |
| Número de clasificación (OCLC) (R) ; Numero de clasificación, CALL (RLIN) (NR) | B-17789 |
| 008 - DATOS DE LONGITUD FIJA--INFORMACIÓN GENERAL | |
| campo de control de longitud fija | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - MENCIÓN DEL TÍTULO | |
| Título | Combination of Suspension Array and Mycelial Growth Assay for Detecting Multiple-Fungicide Resistance in Botrytis cinerea in Hebei Province in China. |
| 490 0# - MENCIÓN DE SERIE | |
| Designación de volumen o secuencia | Plant Disease, 103(6), p.1213-1219, 2019 |
| 520 3# - RESUMEN, ETC. | |
| Sumario, etc. | To provide a high-throughput, efficient, and accurate method to monitor multiple-fungicide resistance of Botrytis cinerea in the field, we used the suspension array, sequencing, and mycelial growth assay in our research. Discriminating-dose bioassays for detecting carbendazim, diethofencarb, boscalid, and iprodione resistance (CarR, DieR, BosR, and IprR, respectively)were used to analyze 257 isolates collected from Hebei Province in China during 2016 and 2017. High resistance frequencies to carbendazim (100percent), diethofencarb (92.08percent), and iprodione (86.59percent)were detected. BosR isolates accounted for 11.67percent of the total. In addition, 103 isolates were randomly selected for phenotype and genotype detection. The high-throughput suspension array was utilized to detect eight genotypes simultaneously, including BenA-E198, BenA-198A, SdhB-H272, SdhB-272Y, BcOS1-I365, BcOS1-365S, erg27-F412, and erg27-412S, which were associated with resistance toward carbendazim or diethofencarb, boscalid, iprodione, and fenhexamid (FenR), respectively. Most of the benzimidazole-resistant isolates (81.55percent)possessed the E198V mutation in the BenA gene. Ninety-three isolates with dual resistance to carbendazim and diethofencarb showed the E198V/K mutation. All BosR isolates carried the H272R mutation in the SdhB gene. The I365S and Q369P+N373S (66.99percent)mutations in the BcOS1 gene were more frequently observed. No mutation was detected in the erg27 gene in Hebei isolates. There were 13 resistance profile phenotypes. Phenotypes with triple resistance were the most common (83.50percent), and CarRDieRBosSIprRFenS was the major type. CarR isolates that carried E198V/K/A were all highly resistant (HR)and only one F200Y mutant was moderately resistant (MR)to carbendazim. Isolates that possessed E198V/K were MR or HR to diethofencarb. BosR isolates that possessed H272R mutation were lowly resistant (LR). IprR isolates were all LR or MR. The distribution of half maximal effective concentrations of CarR isolates with E198V/K mutations and IprR isolates with Q369P+N373S mutations significantly increased from 2016 to 2017. Combined with our observations, a combination method of the high-throughput suspension array and the mycelial growth assay was suggested to accurately monitor multiple resistance of B. cinerea in the field. |
| 700 12 - ENTRADA AGREGADA--NOMBRE PERSONAL | |
| Nombre de persona | Su, Z. |
| 700 12 - ENTRADA AGREGADA--NOMBRE PERSONAL | |
| Nombre de persona | Zhang, X. |
| 700 12 - ENTRADA AGREGADA--NOMBRE PERSONAL | |
| Nombre de persona | Zhao, J. |
| 700 12 - ENTRADA AGREGADA--NOMBRE PERSONAL | |
| Nombre de persona | Wang, W. |
| 700 12 - ENTRADA AGREGADA--NOMBRE PERSONAL | |
| Nombre de persona | Shang, L. |
| 700 12 - ENTRADA AGREGADA--NOMBRE PERSONAL | |
| Nombre de persona | Ma, S. |
| 700 12 - ENTRADA AGREGADA--NOMBRE PERSONAL | |
| Nombre de persona | Mawulipplimi Adzavon, Y. |
| 700 12 - ENTRADA AGREGADA--NOMBRE PERSONAL | |
| Nombre de persona | Mawulipplimi Adzavon, Y. |
| 700 12 - ENTRADA AGREGADA--NOMBRE PERSONAL | |
| Nombre de persona | Weng, M. |
| 700 12 - ENTRADA AGREGADA--NOMBRE PERSONAL | |
| Nombre de persona | Han, X. |
| 700 12 - ENTRADA AGREGADA--NOMBRE PERSONAL | |
| Nombre de persona | Ma, X. |
| 700 12 - ENTRADA AGREGADA--NOMBRE PERSONAL | |
| Nombre de persona | Zhao, Q. |
| 700 12 - ENTRADA AGREGADA--NOMBRE PERSONAL | |
| Nombre de persona | Zhao, |
| 700 12 - ENTRADA AGREGADA--NOMBRE PERSONAL | |
| Nombre de persona | Xie, F. |
| 700 12 - ENTRADA AGREGADA--NOMBRE PERSONAL | |
| Nombre de persona | Yang, L. |
| 856 40 - LOCALIZACIÓN Y ACCESO ELECTRÓNICOS | |
| Identificador Uniforme de Recurso | <a href="https://drive.google.com/file/d/1x7Vpv2vaYWwJwT2-hgDIg3TL9CM_6kWJ/view?usp=drivesdk">https://drive.google.com/file/d/1x7Vpv2vaYWwJwT2-hgDIg3TL9CM_6kWJ/view?usp=drivesdk</a> |
| Nota pública | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ELEMENTOS DE ENTRADA SECUNDARIOS (KOHA) | |
| Fuente de la clasificación o esquema de estantería | |
| Tipo de ítem Koha | REF1 |
| Estatus retirado | Estado de pérdida | Fuente de la clasificación o esquema de estantería | Estado de daño | No para préstamo | Colección | Biblioteca de origen | Biblioteca actual | Ubicación en estantería | Fecha de adquisición | Total de préstamos | Signatura topográfica completa | Visto por última vez | Precio de reemplazo | Tipo de ítem Koha |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CICY | CICY | 25/06/2025 | B-17789 | 25/06/2025 | 25/06/2025 | REF1 |