The PIN (PIN-formed)proteins act as vital carriers, regulating auxin polar transport and playing a crucial role in plant growth and development. Cymbidium ensifolium (Orchidaceae)is a perennial herbaceous plant highly esteemed for its high ornamental value. The lotus-shape flowers of C. ensifolium are favored by consumers for their distinctive flower shape with shorter petals. To deepen our understanding of the members and characteristics of PIN gene family in C. ensifolium, this study performed genome-wide identification and analysis of CePIN members, including their physicochemical properties, protein and gene structures, conserved motifs, phylogenetic evolution, promoter components, and expression patterns. The results revealed a total of 16 PIN gene family members in the genome of C. ensifolium. Expression analysis demonstrated significant differential expression of all 16 CePINs across different tissues. Notably, a close correlation was observed between the expression of CePIN1a and CePIN3 and the formation of lotus-shape flowers in C. ensifolium. These findings provide a foundational understanding for further exploration of CePIN functions and offer valuable insights for studying petal development in C. ensifolium.